Polydeoxyribonucleotide and Shock Wave Therapy Sequence Efficacy in Regenerating Immobilized Rabbit Calf Muscles

This study primarily aimed to investigate the combined effects of polydeoxyribonucleotide (PDRN) and extracorporeal shock wave therapy (ESWT) sequences on regenerative processes in atrophied animal muscles. Thirty male New Zealand rabbits, aged 12 weeks, were divided into five groups: normal saline (Group 1), PDRN 2), ESWT 3), injection before 4), after 5). After 2 weeks cast immobilization, respective treatments administered calf Radial was performed twice weekly. Calf circumference, tibial nerve compound muscle action potential (CMAP), gastrocnemius (GCM) thickness treatment evaluated. Histological immunohistochemical staining, as well Western blot analysis, conducted post-treatment. Staining intensity extent assessed using semi-quantitative scores. Groups 4 5 demonstrated significantly greater GCM thickness, CMAP, fiber cross-sectional area (type I, type II, total) than remaining three groups (p < 0.05), while they did not differ these parameters. 3 showed higher values for all mentioned parameters Group 1 0.05). had greatest ratio vascular endothelial growth factor (VEGF) platelet cell adhesion molecule-1 (PECAM-1) fibers compared other four analysis revealed expression angiogenesis cytokines The combination superior efficacy tissue rabbit models techniques alone or saline. In particular, administering yielded most favorable outcomes specific